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["2020年润基生物科研服务项目及价格表"]
["项目","方法","特点","推荐指数","收费标准","备注"]
["外泌体提取和纯化","外泌体提取","利用聚合物沉淀外泌体，适用于各类标本，可用于核酸提取、ELISA、WB等实验。","★★★☆☆","0.5-2ml：150元/样本                              "]
[{"$nulls":4},"2-10ml：200元/样本                              "]
[{"$nulls":4},"10-50ml：250元/样本"]
[null,"外泌体提取和纯化","利用特殊修饰的树脂，特异结合外泌体脂质双分子层，从而获得较高纯度的外泌体。","★★★★★","0.5-2ml：300元/样本","适用于血清、血浆样本，获得的外泌体纯度高"]
[{"$nulls":4},"2-4ml：400元/样本"]
[{"$nulls":4},">4ml：500元/样本"]
[null,"外泌体提取、浓缩","主要针对大体积样本，利用特殊的树脂快速有效的结合外泌体，从而实现外泌体的浓缩。","★★★★☆","<100ml：300元/样本                           ","适用于细胞培养上清、尿液等大体积样本"]
[{"$nulls":4},"100-200ml：500元/样本                      "]
[{"$nulls":4},">200ml：2.5元/ml"]
[null,"外泌体体磁珠捕获","利用磁珠偶联抗体，实现外泌体的特异捕获，获得高纯度外泌体，可用于包括质谱、测序在内的各类实验。","★★★★★","600元/样本(血清/血浆0.5-2ml；培养液/尿液<20ml)","获得高纯度外泌体或外泌体亚群"]
[{"$nulls":4},"1000元/样本(血清/血浆>2ml；培养液/尿液>20ml)"]
[null,"外泌体SEC纯化","根据尺寸排阻色谱原理，根利用被分离组分的分子大小实现外泌体的分离和纯化，获得的外泌体纯度高，可用于包括质谱、测序在内的各类实验。","★★★★★","500元/样本（血清/血浆<1ml）","样本为培养液或者尿液时，过SEC柱之前需进行样本浓缩"]
[{"$nulls":4},"600元/样本（血清/血浆1-3ml；培养液/尿液<50ml）"]
[{"$nulls":4},"700元/样本（血清/血浆>3ml；培养液/尿液50-150ml）"]
[{"$nulls":4},"800元/样本（培养液/尿液>150ml）"]
["外泌体鉴定、定量","NTA/fNTA","NTA技术可以实现悬浮液中外泌体和微囊泡的直接成像和观察，并可提供高分辨率的粒度分布图表和浓度信息。搭配488nm波长的激光器相应滤光片，可以测量绿色荧光标记的外泌体。","★★★★★","NTA：500元/样本","检出收费，未检出可重新送样，免费再测一次"]
[{"$nulls":4},"fNTA：700元/样本"]
[{"$nulls":4},"Zeta电位：200元/样本（不单独检测，可随NTA或fNTA一起检测）"]
[null,"电镜","直接观察外泌体的大小与结构","★★★★★","1000元/样本","检出收费，未检出不收费或重新送样，免费在测一次"]
[null,"WB","选择合适的抗体（包括CD9，CD63，CD81，Alix，TSG101，Hsp70等），有效检测外泌体生物标志物。","★★★★★","1000元/膜/5-9个样本"]
[{"$nulls":4},"500元/膜/1-4个样本"]
[{"$nulls":4},"800元/膜（客户提供抗体，最多可做9个样本）"]
[null,"ELISA","简单、便捷实现外泌体的捕获和定量；既能完成单一样本多个外泌体标志物的检测，也能完成大量样本单个标志物的检测。","★★★★☆","150元/样本/孔","可根据客户需要进行ELISA板定制"]
["核酸分离、检测","DNA/RNA分离","结合外泌体不同提取方式，可获得高纯度、高产量的外泌体核酸，还可以获得外泌体亚群核酸。","★★★★★","80元/样本","价格为单个样本单次重复价格，不包含内参。引物、探针可免费设计，合成级别为page级，2OD。"]
[null,"DNA/RNA qPCR检测","根据客户提供的检测基因信息，完成引物、探针的设计和合成，并完成qPCR体系的建立、优化和数据分析。","★★★★★","50元/样本"]
[{"$nulls":4},"引物合成1.2元/碱基，Taqman探针1000元/条"]
["外泌体标记和纯化","PKH67（绿）/PKH26（红）         /Dio（绿）/Dil（红）","利用染料高效标记外泌体，通过特殊树脂高效去除染料，纯化标记外泌体。","★★★★★","600元/样本","所需外泌体样本中杂蛋白含量较少"]
["外泌体   粉末化",null,"通过膜亲和与分子排阻分离、纯化外泌体，可根据客户要求定制特定数量/瓶的外泌体粉末，获得的外泌体功能活性均不受影响，并能在4℃长期保存。","★★★★★","50-150ml：15元/ml"]
[{"$nulls":4},"150-250ml：14元/ml"]
[{"$nulls":4},"250-350ml：13元/ml"]
[{"$nulls":4},"350-450ml：12元/ml"]
[{"$nulls":4},">450ml：10元/ml"]
["外泌体服务定制",null,"根据客户的需求，有针对性的完成客户的需求。","★★★★★","另议"]
["说明：本价格为单个样本单个服务价格，多样本及整体服务价格另议。"]

{"sheet":"彩色版","index":1,"n_rows":37,"n_cols":6,"rows_kept":37,"merged":[[0,0,0,5],[2,0,16,0],[2,1,4,1],[2,2,4,2],[2,3,4,3],[2,5,4,5],[5,1,7,1],[5,2,7,2],[5,3,7,3],[5,5,7,5],[8,1,10,1],[8,2,10,2],[8,3,10,3],[8,5,10,5],[11,1,12,1],[11,2,12,2],[11,3,12,3],[11,5,12,5],[13,1,16,1],[13,2,16,2],[13,3,16,3],[13,5,16,5],[17,0,24,0],[17,1,19,1],[17,2,19,2],[17,3,19,3],[17,5,19,5],[21,1,23,1],[21,2,23,2],[21,3,23,3],[21,5,23,5],[25,0,27,0],[25,5,27,5],[26,1,27,1],[26,2,27,2],[26,3,27,3],[29,0,33,0],[29,1,33,1],[29,2,33,2],[29,3,33,3],[36,0,36,5]]}
["2020年润基生物科研服务项目及价格表"]
["项目","方法","特点","推荐指数","收费标准","备注"]
["外泌体提取和纯化","外泌体提取","利用聚合物沉淀外泌体，适用于各类标本，可用于核酸提取、ELISA、WB等实验。","★★★☆☆","0.5-2ml：150元/样本                           "]
[{"$nulls":4},"2-10ml：200元/样本                              "]
[{"$nulls":4},"10-50ml：250元/样本"]
[null,"外泌体提取和纯化","利用特殊修饰的树脂，特异结合外泌体脂质双分子层，从而获得较高纯度的外泌体。","★★★★★","0.5-2ml：300元/样本","适用于血清、血浆样本，获得的外泌体纯度高"]
[{"$nulls":4},"2-4ml：400元/样本"]
[{"$nulls":4},">4ml：500元/样本"]
[null,"外泌体提取、浓缩","主要针对大体积样本，利用特殊的树脂快速有效的结合外泌体，从而实现外泌体的浓缩。","★★★★☆","<100ml：300元/样本                           ","适用于细胞培养上清、尿液等大体积样本"]
[{"$nulls":4},"100-200ml：500元/样本                      "]
[{"$nulls":4},">200ml：2.5元/ml"]
[null,"外泌体体磁珠捕获","利用磁珠偶联抗体，实现外泌体的特异捕获，获得高纯度外泌体，可用于包括质谱、测序在内的各类实验。","★★★★★","600元/样本(血清/血浆0.5-2ml；培养液/尿液<20ml)","获得高纯度外泌体或外泌体亚群"]
[{"$nulls":4},"1000元/样本(血清/血浆>2ml；培养液/尿液>20ml)"]
[null,"外泌体SEC纯化","根据尺寸排阻色谱原理，根利用被分离组分的分子大小实现外泌体的分离和纯化，获得的外泌体纯度高，可用于包括质谱、测序在内的各类实验。","★★★★★","500元/样本（血清/血浆<1ml）","样本为培养液或者尿液时，过SEC柱之前需进行样本浓缩"]
[{"$nulls":4},"600元/样本（血清/血浆1-3ml；培养液/尿液<50ml）"]
[{"$nulls":4},"700元/样本（血清/血浆>3ml；培养液/尿液50-150ml）"]
[{"$nulls":4},"800元/样本（培养液/尿液>150ml）"]
["外泌体鉴定、定量","NTA/fNTA","NTA技术可以实现悬浮液中外泌体和微囊泡的直接成像和观察，并可提供高分辨率的粒度分布图表和浓度信息。搭配488nm波长的激光器相应滤光片，可以测量绿色荧光标记的外泌体。","★★★★★","NTA：500元/样本","检出收费，未检出可重新送样，免费再测一次"]
[{"$nulls":4},"fNTA：700元/样本"]
[{"$nulls":4},"Zeta电位：200元/样本（不单独检测，可随NTA或fNTA一起检测）"]
[null,"电镜","直接观察外泌体的大小与结构","★★★★★","1000元/样本","检出收费，未检出不收费或重新送样，免费在测一次"]
[null,"WB","选择合适的抗体（包括CD9，CD63，CD81，Alix，TSG101，Hsp70等），有效检测外泌体生物标志物。","★★★★★","1000元/膜/5-9个样本"]
[{"$nulls":4},"500元/膜/1-4个样本"]
[{"$nulls":4},"800元/膜（客户提供抗体，最多可做9个样本）"]
[null,"ELISA","简单、便捷实现外泌体的捕获和定量；既能完成单一样本多个外泌体标志物的检测，也能完成大量样本单个标志物的检测。","★★★★☆","150元/样本/孔","可根据客户需要进行ELISA板定制"]
["核酸分离、检测","DNA/RNA分离","结合外泌体不同提取方式，可获得高纯度、高产量的外泌体核酸，还可以获得外泌体亚群核酸。","★★★★★","80元/样本","价格为单个样本单次重复价格，不包含内参。引物、探针可免费设计，合成级别为page级，2OD。"]
[null,"DNA/RNA qPCR检测","根据客户提供的检测基因信息，完成引物、探针的设计和合成，并完成qPCR体系的建立、优化和数据分析。","★★★★★","50元/样本"]
[{"$nulls":4},"引物合成1.2元/碱基，Taqman探针1000元/条"]
["外泌体标记和纯化","PKH67（绿）/PKH26（红）         /Dio（绿）/Dil（红）","利用染料高效标记外泌体，通过特殊树脂高效去除染料，纯化标记外泌体。","★★★★★","600元/样本","所需外泌体样本中杂蛋白含量较少"]
["外泌体   粉末化",null,"通过膜亲和与分子排阻分离、纯化外泌体，可根据客户要求定制特定数量/瓶的外泌体粉末，获得的外泌体功能活性均不受影响，并能在4℃长期保存。","★★★★★","50-150ml：15元/ml"]
[{"$nulls":4},"150-250ml：14元/ml"]
[{"$nulls":4},"250-350ml：13元/ml"]
[{"$nulls":4},"350-450ml：12元/ml"]
[{"$nulls":4},">450ml：10元/ml"]
["外泌体服务定制",null,"根据客户的需求，有针对性的完成客户的需求。","★★★★★","另议"]
[]
["说明：本价格为单个样本单个服务价格，多样本及整体服务价格另议。"]